Microscopie en flux Haute résolution
Nouveau système ImageStreamX
- 12 images par cellule
- 1000 cellules par secondes
- jusqu'à 5 lasers d'excitation
- 20x/40x/60x
La puissance visuelle de la microscopie et la rigueur statistique de la cytométrie de flux sont désormais réunies en une seule plate-forme.
Cette combinaison de l'analyse quantitative des images et de la cytométrie de flux vous permet de mesurer les quantités, la localisation et le mouvement de molécules sur, dans ou entre les cellules. Analyser l'emplacement et la colocalisation de multiples marqueurs sur ou dans les cellules. Quantifier des sous-populations morphologiquement différentes...
Caractéristiques - Applications - Publications scientifiques
Caractéristiques
- 12 images par cellule
- 1000 cellules par secondes
- jusqu'à 5 lasers d'excitation
- 20x/40x/60x
Applications
Translocation moléculaire et signalisation cellulaire
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Application Notes
- Multiplexing Signal Transduction Measurement on the ImageStream for High Content Analysis and High Throughput
(Adobe PDF, 416 KB) - Measurement of nuclear translocation in primary T cells (07-001)
(Adobe PDF, 616 KB) - GFP expression and subcellular compartmentalization in Trypansosomes (05-003)
(Adobe PDF, 469 KB) - Simultaneous measurement of NFkB translocation and apoptosis (05-004)
(Adobe PDF, 584 KB)
- Multiplexing Signal Transduction Measurement on the ImageStream for High Content Analysis and High Throughput
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Papers
- Peroxiredoxin 1 stimulates secretion of proinflammatory cytokines by binding to TLR4
(Link to external website) - NF-E2-related factor 2 regulates the stress response to UVA-1-oxidized phospholipids in skin cells
(Link to external website) - Distinct Regulation of Integrin-Dependent T Cell Conjugate Formation and NF-κB Activation by the Adapter Protein ADAP
(Link to external website) - Lytic Granule Loading of CD8+ T Cells Is Required for HIV-Infected Cell Elimination Associated with Immune Control
(Link to external website) - Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells
(Link to external website) - Autophosphorylation docking site Tyr867 in Mertk allows for dissociation of multiple signaling pathways for phagocytosis of apoptotic cells and downmodulation of LPS-inducible NF-κB transcriptional activation
(Link to external website) - IFN-α Is Not Sufficient to Drive Th1 Development Due to Lack of Stable T-bet Expression
(Link to external website) - Temporal Dissection of T-bet Functions
(Link to external website) - The unexpected effect of cyclosporin A on CD56+CD16- and CD56+CD16+ natural killer cell subpopulations
(Link to external website) - Receptor Cross-Linking on Human Plasmacytoid Dendritic Cells Leads to the Regulation of IFN-α Production
(Link to external website) - Quantitative Measurement of Nuclear Translocation Events using Similarity Analysis of Multispectral Cellular Images Obtained in Flow
(Link to external website)
- Peroxiredoxin 1 stimulates secretion of proinflammatory cytokines by binding to TLR4
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Posters
- A novel method to assess the level of in vivo immunosuppression in transplant patients using Amnis ImageStream technology AAI 2009 (09-002-1-pos)
(Adobe PDF, 379 KB) - Distinct regulatory roles of NF-kB in CpGA and CpGB- induced IFN-gamma production by plasmacytoid dendritic cells AAI 2009 (09-006-1-pos)
(Adobe PDF, 349 KB) - Quantitative assessment of NF-kB activation in heterogeneous cell populations AAI 2009 (09-001-1-pos)
(Adobe PDF, 587 KB) - Measurement of nuclear translocation in primary T cells (07-002-1-pos)
(Adobe PDF, 1.46 MB) - Recent modifications to the ImageStream technology improving its applicability to the study of NFkB activation in a clinical research setting (08-001-1-pos)
(Adobe PDF, 398 KB) - Simultaneous measurement of apoptosis and nuclear translocation events (06-006-1-pos)
(Adobe PDF, 597 KB)
- A novel method to assess the level of in vivo immunosuppression in transplant patients using Amnis ImageStream technology AAI 2009 (09-002-1-pos)
Internalisation
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Application Notes
- Quantitation of gamma-H2AX spots on the ImageStream (09-008)
(Adobe PDF, 373 KB) - Phagocytosis and quantitation of internal zymosan, L. monocytogenes, M. tuberculosis, and Anthrax spores (09-003)
(Adobe PDF, 846 KB) - Kinetics of CpG internalization and sub-cellular organelle co-localization within plasmacytoid dendritic cells (08-001)
(Adobe PDF, 811 KB)
- Quantitation of gamma-H2AX spots on the ImageStream (09-008)
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Papers
- TGF-beta regulates T-cell neurokinin-1 receptor internalization and function
(Link to external website) - Members of the Entamoeba histolytica transmembrane kinase family play non-redundant roles in growth and phagocytosis
(Link to external website) - Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology
(Link to external website) - Clinical and biological significance of CXCR5 expressed by prostate cancer specimens and cell lines
(Link to external website) - Identification of a dendritic cell receptor that couples sensing of necrosis to immunity
(Link to external website) - Image-Based Study of Interferongenic Interactions between Plasmacytoid Dendritic Cells and HSV-Infected Monocyte-Derived Dendritic Cells.
(Link to external website)
- TGF-beta regulates T-cell neurokinin-1 receptor internalization and function
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Posters
- Distinct regulatory roles of NF-kB in CpGA and CpGB- induced IFN-alpha production by plasmacytoid dendritic cells AAI 2009 (09-006-1-pos)
(Adobe PDF, 349 KB) - Intracellular localization and trafficking (07-003-1-pos)
(Adobe PDF, 203 KB) - Kinetics of CpG internalization and sub-cellular organelle co-localization within circulating human plasmacytoid dendritic cells (06-003-1-pos)
(Adobe PDF, 613 KB)
- Distinct regulatory roles of NF-kB in CpGA and CpGB- induced IFN-alpha production by plasmacytoid dendritic cells AAI 2009 (09-006-1-pos)
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Analyse morphologique
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Application Notes
- Identification and Measurement of Bacterial Size using the ImageStream (09-002)
(Adobe PDF, 386 KB) - Oceanography on the ImageStream: Diatoms and other phytoplankton (09-001)
(Adobe PDF, 982 KB) - Measuring shape change in monocytes (09-006)
(Adobe PDF, 243 KB) - FRET analysis of protein-protein interaction and redox sensor folding using the ImageStream (09-005)
(Adobe PDF, 215 KB) - Morphologic and location-based classification of differentiating erythroid lineage cells using the ImageStream (09-004)
(Adobe PDF, 257 KB) - Quantitative analysis of pseudopod formation (05-002)
(Adobe PDF, 1.46 MB)
- Identification and Measurement of Bacterial Size using the ImageStream (09-002)
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Papers
- HIV-1 Nef inhibits ruffles, induces filopodia, and modulates migration of infected lymphocytes
(Link to external website) - Akt inhibition promotes autophagy and sensitizes PTEN-null tumors to lysosomotropic agents
(Link to external website) - Multispectral imaging of hematopoietic cells: Where flow meets morphology
(Link to external website) - Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus
(Link to external website) - Morphological characterization of very small embryonic-like stem cells (VSELs) by ImageStream system analysis
(Link to external website)
- HIV-1 Nef inhibits ruffles, induces filopodia, and modulates migration of infected lymphocytes
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Posters
- Increased body temperature induces lymphocyte lipid raft aggregation AAI 2009 (09-014-1-pos)
(Adobe PDF, 1.49 MB) - Oceanography on the ImageStream imaging flow cytometer: automated quantitative image analysis of plankton, diatoms, and more (08-004-1-pos)
(Adobe PDF, 1.1 MB) - Optimization of isolation and further molecular and functional characterization of SSEA-4+/Oct-4+/CD133+/CXCR4+/Lin neg very small embryonic-like (VSEL) stem cells isolated from umbilical cord blood (08-006-1-pos)
(Adobe PDF, 98 KB)
- Increased body temperature induces lymphocyte lipid raft aggregation AAI 2009 (09-014-1-pos)
Co-localisation
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Application Notes
- Kinetics of CpG internalization and sub-cellular organelle co-localization within plasmacytoid dendritic cells (08-001)
(Adobe PDF, 811 KB) - Analysis of intracellular molecular trafficking (06-002)
(Adobe PDF, 741 KB) - Cell surface co-localization of Complement 3b and Rituximab (04-001)
(Adobe PDF, 464 KB) - FRET analysis of protein-protein interaction and redox sensor folding using the ImageStream (09-005)
(Adobe PDF, 215 KB)
- Kinetics of CpG internalization and sub-cellular organelle co-localization within plasmacytoid dendritic cells (08-001)
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Papers
- Peroxiredoxin 1 stimulates secretion of proinflammatory cytokines by binding to TLR4
(Link to external website) - Unique Biological Properties of Catalytic Domain Directed Human Anti-CAIX Antibodies Discovered through Phage-Display Technology
(Link to external website) - Characterization of the Murine SIRT3 Mitochondrial Localization Sequence and Comparison of Mitochondrial Enrichment and Deacetylase Activity of Long and Short SIRT3 Isoforms
(Link to external website) - NF-E2-related factor 2 regulates the stress response to UVA-1-oxidized phospholipids in skin cells
(Link to external website) - Differential Association of Programmed Death-1 and CD57 with Ex Vivo Survival of CD8+ T Cells in HIV Infection
(Link to external website) - Binding of submaximal C1q promotes complement-dependent cytotoxicity (CDC) of B cells opsonized with anti-CD20 mAbs ofatumumab (OFA) or rituximab (RTX): considerably higher levels of CDC are induced by OFA than by RTX
(Link to external website) - CD44 Deficiency Attenuates Chronic Murine Ileitis
(Link to external website)
- Peroxiredoxin 1 stimulates secretion of proinflammatory cytokines by binding to TLR4
-
Posters
- Increased body temperature induces lymphocyte lipid raft aggregation AAI 2009 (09-014-1-pos)
(Adobe PDF, 1.49 MB) - Distinct regulatory roles of NF-kB in CpGA and CpGB- induced IFN-alpha production by plasmacytoid dendritic cells AAI 2009 (09-006-01-pos)
(Adobe PDF, 349 KB) - Intracellular localization and trafficking (07-003-1-pos)
(Adobe PDF, 203 KB) - Assessment of protein co-localization (06-004-1-pos)
(Adobe PDF, 211 KB) - Quantitative analysis of therapeutic monoclonal antibody localizations to endosomes and lysosomes (06-001-1-pos)
(Adobe PDF, 170 KB) - Kinetics of CpG internalization and sub-cellular organelle co-localization within circulating human plasmacytoid dendritic cells (06-003-1-pos)
(Adobe PDF, 613 KB)
- Increased body temperature induces lymphocyte lipid raft aggregation AAI 2009 (09-014-1-pos)
Interaction cellule-cellule
Application Notes
- Automated quantitation of events with protein localized to the T:APC synapse (08-002)
(Adobe PDF, 917 KB)
Papers
- Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen
(Link to external website) - Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy
(Link to external website) - Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry
(Link to external website) - Distinct Regulation of Integrin-Dependent T Cell Conjugate Formation and NF-κB Activation by the Adapter Protein ADAP
(Link to external website) - Binding of Rituximab, Trastuzumab, Cetuximab, or mAb T101 to Cancer Cells Promotes Trogocytosis Mediated by THP-1 Cells and Monocytes
(Link to external website) - Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer
(Link to external website) - Image-Based Study of Interferongenic Interactions between Plasmacytoid Dendritic Cells and HSV-Infected Monocyte-Derived Dendritic Cells.
(Link to external website)
Posters
- Numbers Matters: Quantitative and Dynamic Analysis of the Formation of an Immunological Synapse using Imaging Flow Cytometry AAI 2009 (09-004-1-pos)
(Adobe PDF, 518 KB) - Tyrosine phosphatase SHP-1 negatively regulates suppressive potential of CD4+CD25+Foxp3+ regulatory T cells AAI 2009 (09-007-1-pos)
(Adobe PDF, 367 KB) - SHP-1 influences resistance of CD4+ T cells to regulatory T cell suppression AAI 2009 (09-009-1-pos)
(Adobe PDF, 375 KB) - Increased body temperature induces lymphocyte lipid raft aggregation AAI 2009 (09-014-1-pos)
(Adobe PDF, 1.49 MB) - Acutely HIVIIIB- Infected CD4+ T Cells and Chronically-Infected HIVIIIB- H9 Cells Preferentially Conjugate and Subsequently Fuse With Plasmacytoid Dendritic Cells AAI 2009 (09-010-1-pos)
(Adobe PDF, 407 KB) - Automated quantitation of events with protein localized to the T:APC synapse (07-001-1-pos)
(Adobe PDF, 648 KB)
Cycle cellulaire et mitose
Application Notes
- Mitotic index multiplexed with apoptotic cell detection (07-002)
(Adobe PDF, 2.97 MB)
Papers
- Optimization of Yeast Cell Cycle Analysis and Morphological Characterization by Multispectral Imaging Flow Cytometry
(Link to external website)
Sélection de publications scientifiques
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Téléchargez Selected Publications
(Adobe PDF, 1.4 MB)
Dendritic cells control T cell tonic signaling required for responsiveness to foreign antigen
Hochweller K, Wabnitz GH, Samstag Y, Suffner J, Hämmerling GJ, Garbi N.
PNAS (2010) 107(13):5931-6.
Characterization of the Murine SIRT3 Mitochondrial Localization Sequence and Comparison of Mitochondrial Enrichment and Deacetylase Activity of Long and Short SIRT3 Isoforms
Bao, J., Lu, Z., Joseph, J.J., Carabenciov, D., Dimond, C.C., Pang, L., Samsel, L., McCoy, J.P., Leclerc, J., Nguyen, P., Gius, D., and Sack, M.N.
Journal of Cellular Biochemistry (2010) 9999:1-10
Unique biological properties of catalytic domain directed human anti-CAIX antibodies discovered through phage-display technology
Xu C, Lo A, Yammanuru A, Tallarico AS, Brady K, Murakami A, Barteneva N, Zhu Q, Marasco WA.
PLoS One. 2010 Mar 10;5(3):e9625
Members of the Entamoeba histolytica transmembrane kinase family play non-redundant roles in growth and phagocytosis
Buss, S.N., Hamano, S., Vidrich, A., Evans, C., Zhang, Y., Crasta, O.R., Sobral, B.W., Gilchrist, C.A., Petri Jr., W.A.
International Journal for Parasitology (2010) Jan 18,( Epub ahead of print)
TGF-beta regulates T-cell neurokinin-1 receptor internalization and function
Beinborn, M., Blum, A., Hang, L., Setiawan, T., Schroeder, J.C., Stoyanoff, K., Leung, J., and Weinstock, J.V.
Proc Natl Acad Sci. (2010) Feb. 16 [Epub ahead of print]
HIV-1 Nef inhibits ruffles, induces filopodia and modulates migration of infected lymphocytes
Nobile, C., Rudnicka, D., Hasan, M., Aulner, N., Porrot, F., Machu, C., Renaud, O., Prevost, M.C., Hivroz, C., Schwartz, O., Sol-Foulon, N.
Journal of Virology (2009) Dec. 16 [Epub ahead of print]
Peroxiredoxin 1 Stimulates Secretion of Proinflammatory Cytokines by Binding to TLR4
Riddell, J.R., Wang, X., Minderman, H., O.Gollnick, S.
Journal of Immunology (2009) 184: 1022-30
Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy
Hosseini, B. H., Louban, I., Djandji, D., Wabnitz, G. H., Deeg, J., Bulbuc, N., Samstag, Y., Gunzer, M., Spatz, J.,P.,Hammerling, G. J.
Proc Natl Acad Sci. (2009)106(42):17852-7. Epub 2009 Oct 12.
Numbers matter: quantitative and dynamic analysis of the formation of an immunological synapse using imaging flow cytometry
Fariyal Ahmed, Sherree Friend, Thaddeus George, Natasha Barteneva, and Judy Leiberman
Journal of Immunological Methods (2009) 125(10):2288-95
Clinical and biological significance of CXCR5 expressed by prostate cancer specimens and cell lines
Singh S, Singh R, Singh UP, Rai SN, Novakovic KR, Chung LW, Didier PJ, Grizzle WE, Lillard JW Jr.
International Journal of Cancer (2009) 125(10):2288-95
Statins inhibit NK-cell cytotoxicity by interfering with LFA-1-mediated conjugate formation
Patrick C. Raemer, Kristine Kohl and Carsten Watzl
European Journal of Immunology (2009) 39(6):1456-65
Retinoic acid induces nuclear accumulation of Raf1 during differentiation of HL-60 cells
James Smith, Rodica P. Bunaciu, Gudrun Reiterer, David Coder, Thaddeus George, Michael Asaly, Andrew Yen
Experimental Cell Research (2009)315(13):2241-48
Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer
Michael Z Gilcrease, Xiao Zhou, Xiaolin Lu, Wendy A Woodward, Brian E Hall, and Phillip J Morrissey
Journal of Experimental & Clinical Cancer Research (2009) 28:67-77
The Urokinase Plasminogen Activator Receptor Promotes Efferocytosis of Apoptotic Cells
Veera D’mello, Sukhwinder Singh, Yi Wu, and Raymond B. Birge
The Journal of Biological Chemistry (2009) 284(25):17030–17038
NF-E2-related factor 2 regulates the stress response to UVA-1-oxidized phospholipids in skin cells
Florian Gruber, Herbert Mayer, Barbara Lengauer, Veronika Mlitz, John M. Sanders, Alexandra Kadl, Martin Bilban, Rainer de Martin Oswald Wagner, Thomas W. Kensler, Masayuki Yamamoto, Norbert Leitinger, and Erwin Tschachler
The FASEB Journal (Ahead of Print) Published online August 31, 2009
Differential Association of Programmed Death-1 and CD57 with Ex Vivo Survival of CD8+ T Cells in HIV Infection
Constantinos Petrovas, Benjamin Chaon, David R. Ambrozak, David A. Price, J. Joseph Melenhorst, Brenna J. Hill, Christof Geldmacher, Joseph P. Casazza,
Pratip K. Chattopadhyay, Mario Roederer, Daniel C. Douek, Yvonne M. Mueller, Jeffrey M. Jacobson, Viraj Kulkarni, Barbara K. Felber, George N. Pavlakis, Peter D. Katsikis, and Richard A. Koup
The Journal of Immunology, (2009) 183(2):1120-32
Binding of Submaximal C1q Promotes Complement-Dependent Cytotoxicity (CDC) of B Cells Opsonized with Anti-CD20 mAbs Ofatumumab (OFA) or Rituximab (RTX): Considerably Higher Levels of CDC Are Induced by OFA than by RTX
Andrew W. Pawluczkowycz, Frank J. Beurskens, Paul V. Beum, Margaret A. Lindorfer, Jan G. J. van de Winkel, Paul W. H. I. Parren, and Ronald P. Taylor
The Journal of Immunology, (2009) 183: 749–758
Identification of a dendritic cell receptor that couples sensing of necrosis to immunity
David Sancho, Olivier P. Joffre, Anna M. Keller, Neil C. Rogers, Dolores Martínez, Patricia Hernanz-Falcón, Ian Rosewell & Caetano Reis e Sousa
Nature (2009) 458, 899-903
Distinct Regulation of Integrin-Dependent T Cell Conjugate Formation and NF-κB Activation by the Adapter Protein ADAP
Brandon J. Burbach, Rupa Srivastava, Ricardo B. Medeiros, William E. O'Gorman, Erik J. Peterson and Yoji Shimizu
The Journal of Immunology (2008) 181: 4840-4851
Binding, Internalization, and Antigen Presentation of Vaccine-Loaded Nanoengineered Capsules in Blood
Robert De Rose, Alexander N. Zelikin, Angus P. R. Johnston, Amy Sexton, Siow-Feng Chong, Christina Cortez, William Mulholland, Frank Caruso, Stephen J. Kent
Advanced Materials (2008) 20:4698-4703
Akt inhibition promotes autophagy and sensitizes PTEN-null tumors to lysosomotropic agentsMichael Degtyarev, Ann De Mazière, Christine Orr, Jie Lin, Brian B. Lee, Janet Y. Tien, Wei W. Prior, Suzanne van Dijk, Hong Wu, Daniel C. Gray, David P. Davis, Howard M. Stern, Lesley J. Murray, Klaus P. Hoeflich, Judith Klumperman, Lori S. Friedman, and Kui Lin
The Journal of Cell Biolog (2008) Vol. 183, No. 1, 101-116
Interferon regulatory factor 7-mediated responses are defective in cord blood plasmacytoid dendritic cells
Danis, B., George, T. C., Goriely, S., Dutta, B., Renneson, J., Gatto, L., Fitzgerald-Bocarsly, P., Marchant, A., Goldman, M., Willems, F., DeWit, D.
European Journal of Immunology (2008) Vol. 38, No 2, 507-17
CD44 Deficiency Attenuates Chronic Murine Ileitis
Colm B. Collins, et. al.
Gastroenterology (2008) 135:1993-2002
Lytic Granule Loading of CD8+ T Cells Is Required for HIV-Infected Cell Elimination Associated with Immune Control
Stephen A. Migueles, et. al.
Immunity (2008) 29:1009-1021
Binding of Rituximab, Trastuzumab, Cetuximab, or mAb T101 to Cancer Cells Promotes Trogocytosis Mediated by THP-1 Cells and Monocytes
Paul V. Beum, David A. Mack, Andrew W. Pawluczkowycz, Margaret A. Lindorfer, and Ronald P. Taylor
The Journal of Immunology (2008) 181(11):8120-8132
Deregulation of c-Myc Confers Distinct Survival Requirements for Memory B Cells, Plasma Cells, and Their Progenitors
Sefat E. Khuda, William M. Loo, Siegfried Janz, Brian Van Ness, and Loren D. Erickson=
The Journal of Immunology (2008) 181(11):7537-7549
Multispectral imaging of hematopoietic cells: Where flow meets morphology
Kathleen E. McGrath, Timothy P. Bushnell, James Palis
Journal of Immunological Methods (2008) 336:91-97
Quantitative image based apoptotic index measurement using multispectral imaging flow cytometry: a comparison with standard photometric methods
Shannon Henery, Thaddeus George, Brian Hall, David Basiji, William Ortyn, Philip Morrissey
Apoptosis (2008) 13:1054-1063
Optimization of Yeast Cell Cycle Analysis and Morphological Characterization by Multispectral Imaging Flow Cytometry
Meredith E. K. Calvert, Joanne A. Lannigan, Lucy F. Pemberton
Cytometry Part A (2008) 73A:825-833
Autophosphorylation docking site Tyr867 in Mertk allows for dissociation of multiple signaling pathways for phagocytosis of apoptotic cells and downmodulation of LPS-inducible NF-κB transcriptional activation
Nitu Tibrewal, Yi Wu, Veera D’mello, Reiko Akakura, Thaddeus C. George, Brian Varnum, Raymond B. Birge
Journal of Biological Chemistry (2008) 283(6):3618-3627
Image-Based Study of Interferongenic Interactions between Plasmacytoid Dendritic Cells and HSV-Infected Monocyte-Derived Dendritic Cells
Nicholas J. Megjugorac, Evan S. Jacobs, Alexander G. Izaguirre, Thaddeus C. George, Gunjan Gupta, Patricia Fitzgerald-Bocarsly
Immunological Investigations (2007) 36:739–761
Enucleation of primitive erythroid cells generates a transient population of “pyrenocytes” in the mammalian fetus
Kathleen E. McGrath, Paul D. Kingsley, Anne D. Koniski, Rebecca L. Porter, Timothy P. Bushnell, James Palis
Blood (2008) 111:2409-2417.
Morphological characterization of very small embryonic-like stem cells (VSELs) by ImageStream system analysis
Ewa K. Zuba-Surma, Magdalena Kucia, Ahmed Abdel-Latif, Buddhadeb Dawn, Brian Hall, Rajesh Singh, James W. Lillard, Jr., Mariusz Z. Ratajczak
Journal of Cellular and Molecular Medicine (2007) 12(1):292-303
IFN-α Is Not Sufficient to Drive Th1 Development Due to Lack of Stable T-bet Expression
Hilario J. Ramos, Ann M. Davis, Thaddeus C. George, J. David Farrar.
The Journal of Immunology (2007) 179:3792–3803
Cellular Image Analysis and Imaging by Flow Cytometry
David A. Basiji, William E. Ortyn, Luchuan Liang, Vidya Venkatachalam and Philip Morrissey
Clinics in Laboratory Medicine (2007) 27:653-670
Temporal Dissection of T-bet Functions
Jennifer L. Matsuda, Thaddeus C. George, James Hagman, Laurent Gapin
The Journal of Immunology (2007) 178:3457-3465
Intracellular Kaposi’s Sarcoma-Associated Herpesvirus Load Determines Early Loss of Immune Synapse Components
Laura A. Adang, Costin Tomescu, Wai K. Law, Dean H. Kedes
Journal of Virology (2007) 81(10):5079-5090
Extended Depth of Field Imaging for High Speed Cell Analysis
William E. Ortyn, David J. Perry, Vidya Venkatachalam, Luchuan Liang, Brian E. Hall, Keith Frost, David A. Basiji
Cytometry Part A (2007) 71A:215-231
Autophagy-Dependent Viral Recognition by Plasmacytoid Dendritic Cells
Heung Kyu Lee, Jennifer M. Lund, Balaji Ramanathan, Noboru Mizushima, Akiko Iwasaki
Science (2007) 315:1398-1401
The unexpected effect of cyclosporin A on CD56+CD16- and CD56+CD16+ natural killer cell subpopulations
Hongbo Wang, Bartosz Grzywacz, David Sukovich, Valarie McCullar, Qing Cao, Alisa B. Lee, Bruce R. Blazar, David N. Cornfield, Jeffrey S. Miller, Michael R. Verneris
Blood (2007) 110:1530-1539
Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging flow cytometer
Paul V. Beum, Margaret A. Lindorfer, Brian E. Hall, Thaddeus C. George, Keith Frost, Philip J. Morrissey, Ronald P. Taylor
Journal of Immunological Methods (2006) 317:90-99
Receptor Cross-Linking on Human Plasmacytoid Dendritic Cells Leads to the Regulation of IFN-α Production
Stacey L. Fanning, Thaddeus C. George, Di Feng, Steven B. Feldman, Nicholas J. Megjugorac, Alexander G. Izaguirre, and Patricia Fitzgerald-Bocarsly
The Journal of Immunology (2006) 177:5829-5839
Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell Analysis of Kaposi’s Sarcoma-Associated Herpesvirus Infection
Laura A. Adang, Christopher H. Parsons, Dean H. Kedes
Journal of Virology (2006) 80:10073-10082
Sensitivity Measurement and Compensation in Spectral Imaging
William E. Ortyn, Brian E. Hall, Thaddeus C. George, Keith Frost, David A. Basiji, David J. Perry, Cathleen A. Zimmerman, David Coder, Philip J. Morrissey
Cytometry Part A (2006) 69A:852-862
Quantitative Measurement of Nuclear Translocation Events using Similarity Analysis of Multispectral Cellular Images Obtained in Flow
Thaddeus C. George, Stacey L. Fanning, Patricia Fitzgerald-Bocarsly, Ricardo B. Medeiros, Sarah Highfill, Yoji Shimizu, Brian E. Hall, Keith Frost, David A.Basiji, William E. Ortyn, Philip J. Morrissey, David H. Lynch
Journal of Immunological Methods (2006) 311:117-129
