Imagerie et Analyse de Cellules en Flux
Système ImageStream
La puissance visuelle de la microscopie et la rigueur statistique de la cytométrie de flux sont désormais réunies en une seule plate-forme.
Cette combinaison de l'analyse quantitative des images et de la cytométrie de flux vous permet de mesurer les quantités, la localisation et le mouvement de molécules sur, dans ou entre les cellules. Analyser l'emplacement et la colocalisation de multiples marqueurs sur ou dans les cellules. Quantifier des sous-populations morphologiquement différentes...
Caractéristiques - Applications - Publications scientifiques
Caractéristiques
- 6 images par cellule: fond sombre, fond clair et 4 mesures de fluorescence
- 10 000 cellules par minute
- plus de 200 paramètres par cellules
- volume d'échantillon: 40-200 µl
- taille de pixel: 0.5 micron x 0.5 micron
Applications
Apoptose
Accurate and Reliable Multiparametric Analysis of Apoptosis
Distinguishing modes of cell death using ImageStream Multispectral Imaging Cytometry
Advances in the identification of apoptotic cells
Quantitation of Apoptosis by Nuclear Fragmentation
Translocation nucléaire
Quantitation of Nuclear Translocation Events Using ImageStream Multispectral Imaging Cytometry
Simultaneous Measurement of Nf-kB Translocation and Apoptosis with the ImageStream Multispectral Cell Imaging System
Classification des Leucocytes
Multiplexed Analysis of Cells and Beads with the ImageStream Imaging Flow Cytometer
Analyse du Cycle Cellulaire
Mitotic Index Multiplexed with Apoptotic Cell Detection
Co-localisation de marqueurs
Analyse quantitative de la morphologie cellulaire
Quantitative Analysis of Pseudopod Formation with the ImageStream® Cell Imaging System
Trafic moléculaire intracellulaire
Analysis of Intracellular Molecular Trafficking using ImageStream® Multispectral Imaging
Quantification de l'expression de gènes
Publications scientifiques
IL-13 induces the expression of the alternative activation marker Ym1 in a subset of testicular macrophages.
Katarzyna Maresz, Eugene D. Ponomarev, Natasha Barteneva, Yanping Tan, Monica K. Manna, Bonnie N. Dittel
J. Reprod. Immunol. (2008), doi:10.1016/j.jri.2008.01.001.
Hunt for pluripotent stem cell -- Regenerative medicine search for almighty cell.
Mariusz Z. Ratajczak, Ewa K. Zuba-Surma, Marcin Wysoczynski, Wu Wan, Janina Ratajczak, Wojciech Wojakowski, Magda Kucia
Journal of Autoimmunity 30 (2008) 151-162.
Microscopic imaging techniques for drug discovery.
Andrew Bullen
Nat Rev Drug Discov. (2008) Jan;7(1):54-67.
The oncoprotein H-RasV12 increases mitochondrial metabolism.
Sucheta Telang, Andrew N Lane, Kristin K Nelson, Sengodagounder Arumugam and Jason Chesney
Cancer Molecular Cancer (2007) 6:77.
Oncogenic CARD11 Mutations in Human Diffuse Large B Cell Lymphoma.
Georg Lenz, R. Eric Davis, Vu N. Ngo, Lloyd Lam, Thaddeus C. George,George W. Wright, Sandeep S. Dave, Hong Zhao, Weihong Xu, Andreas Rosenwald, German Ott,Hans Konrad Muller-Hermelink, Randy D. Gascoyne, Joseph M. Connors, Lisa M. Rimsza, Elias Campo, Elaine Jaffe, Jan Delabie, EB Smeland, RFisher, WC Chan, L M. Staudt
SCIENCE (2008) DOI: 10.1126/science.1153629
Cyclooxygenase-2 independent effects of cyclooxygenase-2 inhibitors on oxidative stress and intracellular glutathione content in normal and malignant human B-cells.
Elizabeth P. Ryan, Timothy P. Bushnell, Alan E. Friedman, Irfan Rahman and Richard P. Phipps Cancer Immunol Immunother (2008) 57:347–358.
“Decoding the Dots”: The ImageStream System as a Novel and Powerful Tool for Flow Cytometric Analysis .
Ewa K. Zuba-Surma, Magdalena Kucia, and Mariusz Z. Ratajczak
Cent. Eur. J. Biol. DOI: 10.2478/s11535-007-0044-8
Entamoeba histolytica Phagocytosis of Human Erythrocytes Involves PATMK, a Member of the Transmembrane Kinase Family.
Douglas R. Boettner, Christopher D. Huston, Alicia S. Linford, Sarah N. Buss, Eric Houpt, Nicholas E. Sherman, William A. Petri, Jr.
PLOS (2008) 4:1-121
Image-Based Study of Interferongenic Interactions between Plasmacytoid Dendritic Cells and HSV-Infected Monocyte-Derived Dendritic Cells.
Nicholas J. Megjugorac; Evan S. Jacobs; Alexander G. Izaguirre; Thaddeus C. George; Gunjan Gupta; Patricia Fitzgerald-Bocarsly JImmunological Inviestigations (2007) 36:739–761
Autophosphorylation docking site Tyr867 in Mertk allows for dissociation of multiple signaling pathways for phagocytosis of apoptotic cells and down-modulation of LPSinducible NF-κB transcriptional activation.
Nitu Tibrewal, Yi Wu,, Veera D’ mello, Reiko Akakura, Thaddeus C. George, Brian Varnum, and Raymond B. Birge Journal of Biological Chemistry (2007) doi/10.1074/jbc.M706906200
Morphological characterization of very small embryonic- like stem cells (VSELs) by image stream system analysis.
Ewa K. Zuba-Surma, Magdalena Kucia, Ahmed Abdel-Latif, Buddhadeb Dawn, Brian Hall, Rajesh Singh, James W. Lillard, Jr. and Mariusz Z. Ratajczak Journal of Cellular and Molecular Medicine(2007) Postprint 10.1111/j.1582-4934.2007.00154.x
Enucleation of primitive erythroid cells generates a transient population of "pyrenocytes" in the mammalian fetus.
Kathleen E. McGrath, Paul D. Kingsley, Anne D Koniski, Rebecca L. Porter, Timothy P. Bushnell and James Palis Blood (2007) doi:10.1182/blood-2007-08-107581.
BAI1 is an engulfment receptor for apoptotic cells upstream of the ELMO/Dock180/Rac module. Daeho Park, Annie-Carole Tosello-Trampont, Michael R. Elliott, Mingjian Lu, Lisa B. Haney, Zhong Ma, Alexander L. Klibanov, James W. Mandell and Kodi S. Ravichandran
Nature (2007) DOI:10.1038/nature06329.
A novel multiparametric flow cytometry-based cytotoxicity assay simultaneously immunophenotypes effector cells: Comparisons to a 4 h 51Cr-release assay.
G.G. Kim, V.S. Donnenberg, A.D. Donnenberg, W. Gooding , T.L. Whiteside
Journal of Immunological Methods (2007) 325: 51-66..
Wnt signaling promotes hemato-endothelial cell development from human embryonic stem
cells.
Petter S. Woll, Julie K. Morris, Matt S. Painschab, Rebecca K. Marcus, Aimee D. Kohn,
Travis L. Biechele, Randall T. Moon, and Dan S. Kaufman
Blood (2007) DOI 10.1182/blood-2007-04-084186 .
Tumorigenic Epithelial Stem Cells and Their Normal Counterparts
V.S. Donnenberg, J.D. Luketich, R.J. Landreneau, J.A. DeLoia, P. Basse, A.D. Donnenberg
Ernst Schering Foundation Symposium Proceedings, 2007 Vol. 5, pp. 245–263.
IFN-alpha Is Not Sufficient to Drive Th1 Development Due to Lack of Stable T-bet Expression.
Hilario J. Ramos, Ann M. Davis, Thaddeus C. George, and J. David Farrar
The Journal of Immnology (2007) 179: 3792–3803.
Rapid and Selective Death of Leukemia Stem and Progenitor cells Induced by the Compound
4-benzyl, 2-methyl, 1,2,4-thiadiazolidine, 3,5 dione (TDZD-8).
Monica L. Guzman, Xiaojie Li, Cheryl A. Corbett, Randall M. Rossi, Timothy Bushnell, Jane L. Liesveld, Josée Hébert, Fay Young, Craig T. Jordan.
Blood (2007) DOI 10.1182/blood-2007-05-088815.
Cellular Image Analysis and Imaging by Flow Cytometry.
David A. Basiji, William E. Ortyn, Luchuan Liang, Vidya Venkatachalam and Philip Morrissey
Clinics in Laboratory Medicine (2007) 27:653-70.
The Unexpected Effect of Cyclosporin A on CD56+CD16 and CD56+CD16+ Natural Killer Cell Subpopulations.
Wang H, Grzywacz B, Sukovich D, McCullar V, Cao Q, Lee AB, Blazar BR, Cornfield DN, Miller JS, Verneris MR.
Blood (2007) 110:1530-39
The Flow of Cytometry into Systems Biology.
John P Nolan and Loretta Yang
Briefings in Functional Genomics and Proteomics(2007) doi:10.1093/bfgp/elm011.
Aryl Hydrocarbon Receptor Activation during Influenza Virus Infection Unveils a Novel Pathway of IFN- Production by Phagocytic Cells.
Haley Neff-LaFord, Sabine Teske, Timothy P. Bushnell, and B. Paige Lawrence
Journal of Immunology (2007) 179: 247-55.
NKT Cell Activation Mediates Neutrophil IFN-g Production and Renal Ischemia-Reperfusion Injury.
Li Li, Liping Huang, Sun-sang J. Sung, Peter I. Lobo, Michael G. Brown, Randall K. Gregg, Victor H. Engelhard and Mark D. Okusa
Journal of Immunology (2007) 178: 5899-911.
The Doublecortin-expressing Population in the Developing and Adult Brain Contains Multipotential Precursors in Addition to Neuronal-lineage Cells.
Tara L. Walker, Takahiro Yasuda, David J. Adams, and Perry F. Bartlett
Journal of Neuroscience (2007) 27: 3734-42
Temporal Dissection of T-bet Functions
Jennifer Matsuda, Thaddeus C. George, James Hagman and Laurent Gapin
Journal of Immunology (2007) 178: 3457-65.
Regulation of NF-kB Activation in T Cells via Association with the Adaptor Proteins ADAP and CARMA1
Ricardo Madeiros, Brandon Burbach, Kirsten Mueller, Ripa Srivastava, James J Moon, Sarah Highfill, James J Peterson and Yoji Shimizu
Science (2007) 316: 754-8.
Extended depth of field imaging for high speed cell analysis
William E Ortyn, David J Perry, Vidya Venkatachalam, Luchuan Liang, Brian E Hall, Keith Frost, David A Basiji
Cytometry, Part A (2007) Apr; 71(4):215-31
Autophagy-Dependent Viral Recognition by Plasmacytoid Dendritic Cells
Heung Kyu Lee, Jennifer M. Lund, Balaji Ramanathan, Noboru Mizushima, Akiko Iwasaki
Science (2007) 315: 1398-1401
Quantitative analysis of protein co-localization on B cells opsonized with rituximab and complement using the ImageStream multispectral imaging cytometer
Paul V. Beum, Margaret A. Lindorfer, Brian E. Hall, Thaddeus C. George, Keith Frost, Philip J. Morrissey, Ronald P. Taylor.
Journal of Immunological Methods (2006) 317:90-99
Receptor Cross-Linking on Human Plasmacytoid Dendritic Cells Leads to the Regulation of IFN-Alpha Production
Stacey L. Fanning, Thaddeus C. George, Di Feng, Steven B. Feldman, Nicholas J. Megjugorac, Alexander G. Izaguirre, and Patricia Fitzgerald-Bocarsly.
Journal of Immunology (2006) 177: 5829-5839
Asynchronous Progression through the Lytic Cascade and Variations in Intracellular Viral Loads Revealed by High-Throughput Single-Cell Analysis of Kaposi's Sarcoma-Associated Herpesvirus Infection
Laura A. Adang, Christopher H. Parsons, and Dean H. Kedes.
Journal of Virology (2006) 80:10073-10082
Sensitivity measurement and compensation in spectral imaging
WE Ortyn, BE Hall, TC George, K Frost, DA Basiji, DJ Perry, CA Zimmerman, D Coder, PJ Morrissey.
Cytometry (2006) PartA 69A: 852-862)
KSHV targets multiple leukocyte lineages during long-term productive infection in NOD/SCID mice
CH Parsons, LA Adang, J Overdevest, CM O'Connor, JR Taylor Jr, D Camerini and DH Kedes. Journal of Clinical Investigation (2006) 116: 1963-73
Features of Medullary Thymic Epithelium Implicate postnatal Development in Maintaining Epithelial Heterogeneity and Tissue-Restricted Antigen Expression
GO Gillard, AG Farr
Journal of Immunology (2006), 176: 5815-5824
Quantitative measurement of nuclear translocation events using similarity analysis of multispectral cellular images obtained in flow.
T.C. George, S.L. Fanning, P. Fitzgerald-Bocarsly, R.B. Medeiros , S.Highfill, Y.Shimizu, B.E. Hall, K.Frost, D.A.Basiji, W.E. Ortyn, P.J. Morrissey, D.H. Lynch
Journal of Immunological Methods (2006) Apr 20;311(1-2):117-29.
Multispectral Imaging in Flow: A Technique for Advanced Cellular Studies in Flow Cytometry for Biotechnology
Larry A. Sklar, Editor. Oxford University Press, 2005.
Flow Cytometry, Smaller and Better.
L. Bonetta.
Nature Methods (2005) 2(10), 785-95.
Cutting Edge: FADD Is Not Required for Antigen Receptor-Mediated NF-kB Activation.
A. F. Arechiga, B.D. Bell, J. C. Solomon, I.H. Chu, C.L. Dubois, B. E. Hall, T.C. George, D.M. Coder, C.M. Walsh.
Journal of Immunological Methods (2005) 175, 7800-04.
Distinguishing Modes of Cell Death Using the ImageStream Multispectral Imaging Spectrometer.
T.C. George, D.A. Basiji, B.E. Hall, D.H. Lynch, W.E. Ortyn, D.J. Perry, M.J. Seo, C.A. Zimmerman, P.J. Morrissey
Cytometry (2004) 59A, 237-45.
